Terminator

Part:BBa_K4451001:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


L2U5H11


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BBa_K4451001 (L2U5H11) is a synthetic termination signal characterised by Chen et al. (2013) and included in the EcoFlex MoClo kit (Moore et al., 2016). Tschirhart et al., 2019 showed that L2U5H11 is functional in both E. coli DH5α and V. natriegens ATCC14048 (type strain).

Source

https://doi.org/10.1021/acssynbio.6b00031

References

Chen, Y.-J. et al. Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat. Methods 10, 659–664 (2013).

Moore, S. J. et al. EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology. ACS Synth. Biol. 5, 1059–1069 (2016).

Tschirhart, T. et al. Synthetic Biology Tools for the Fast-Growing Marine Bacterium Vibrio natriegens. ACS Synth. Biol. 8, 2069–2079 (2019).